Fig. 4
From: Stereology with OPEN-Stereo: low-cost, accessible, and accurate cellular quantification
Screenshot of the OPEN-Stereo GUI running the stereology counting program. Once the ROI is defined, and the user has selected the high magnification, (a) pressing the “Run OPEN-Stereo” button starts the stereology point counting program. The live screen updates in the GUI along with a complete outline of the hippocampal CA1 region on the right-hand side. (b) The current location is marked on the outline along with all counting locations. (c) Information is also provided at the top of the GUI, including that the image is “LIVE,” the current counting window, the number of points counted, the current site, and the total number of points counted. (d) Z-positioning panel for identifying the ”top” and ”bottom” of the tissue section, current z position, and the measured section thickness ”z-rel”.
