Table 1 .
From: Stereology with OPEN-Stereo: low-cost, accessible, and accurate cellular quantification
Case number | Antibody | Brain region | Every nth section | Counting frame | Sampling grid area | Raw count | Estimated number of cells | % Congruence | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Commercial | Open-Stereo | Commercial | Open-Stereo | Commercial | Open-Stereo | Commercial | Open-Stereo | |||||
1 | NeuN | CA1 | 12 | 30 × 30 | 30 × 30 | 150 × 150 | 150 × 150; k=5 | 159 | 153 | 86127 | 82620 | 95.8 |
2 | NeuN | CA1 | 12 | 30 × 30 | 30 × 30 | 150 × 150 | 150 × 150; k=5 | 132 | 137 | 74551 | 77268 | 96.4 |
3 | NeuN | CA1 | 12 | 30 × 30 | 30 × 30 | 150 × 150 | 150 × 150; k=5 | 149 | 159 | 84699 | 90830 | 93.2 |
4 | NeuN | CA1 | 12 | 30 × 30 | 30 × 30 | 150 × 150 | 150 × 150; k=5 | 164 | 136 | 92349 | 76704 | 81.5 |
5 | NeuN | CA3 | 12 | 83 × 83 | 30 × 30 | 200 × 200 | 180 × 180; k=6 | 107 | 116 | 92513 | 95213 | 97.1 |
6 | NeuN | CA3 | 12 | 83 × 83 | 30 × 30 | 200 × 200 | 180 × 180; k=6 | 498 | 106 | 72218 | 93416 | 74.4 |
7 | NeuN | CA3 | 12 | 83 × 83 | 30 × 30 | 200 × 200 | 180 × 180; k=6 | 421 | 70 | 60168 | 62294 | 96.5 |
8 | NeuN | CA3 | 12 | 50 × 50 | 30 × 30 | 200 × 200 | 180 × 180; k=6 | 130 | 97 | 106579 | 87160 | 80 |
9 | ChAT | MS | 6 | 85 × 85 | 40 × 40 | 140 × 140 | 80 × 80; k=2 | 102 | 69 | 2526 | 2070 | 80.2 |
10 | ChAT | MS | 6 | 85 × 85 | 40 × 40 | 140 × 140 | 80 × 80; k=2 | 80 | 79 | 2073 | 2370 | 86.6 |
11 | ChAT | MS | 6 | 85 × 85 | 40 × 40 | 140 × 140 | 80 × 80; k=2 | 149 | 67 | 3529 | 2665 | 72.1 |
12 | ChAT | MS | 6 | 85 × 85 | 40 × 40 | 140 × 140 | 80 × 80; k=2 | 103 | 70 | 2319 | 2100 | 90.1 |
