Fig. 2 | Scientific Reports

Fig. 2

From: Suspension cultivation of mosquito cell lines for the production of the mosquito-borne flavivirus Usutu virus in a stirred-tank bioreactor

Fig. 2

The adaptation of C6/36 cells to serum-free suspension conditions using EX-CELL medium. (A) Adaptation of C6/36 cells from an adherent culture by seeding cells at 0.7 × 106 cells/mL in 10 mL. After two passages (grey) the culture was split into two duplicate cultures (black). (B) Revival of a frozen cell bank of suspension-adapted C6/36 cells. The first two passages are from a single culture and represent recovery (grey). The cell concentration of subsequent passages represent the average of two replicate cultures (black). The average and standard deviation of the duplicate cultures are shown (n = 2). The dotted line shows a passaging event. (C) Characterization of C6/36 growth in serum-free suspension (n = 3). (D) Example image of C6/36 cell morphology in EX-CELL and L-15 medium after detachment from an adherent culture. Error bars indicate the standard error of the mean.

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