Fig. 3 | Scientific Reports

Fig. 3

From: Suspension cultivation of mosquito cell lines for the production of the mosquito-borne flavivirus Usutu virus in a stirred-tank bioreactor

Fig. 3

The adaptation of U4.4 cells to suspension cultivation in EX-CELL medium (A) U4.4 cells were adapted to suspension culture in EX-CELL medium from an adherent culture grown in L-15 medium by inoculating at 0.7 × 106 cells/mL in 10 mL. The first two passages are a single culture (grey), which was split into two duplicate cultures (black). The dotted line shows a passaging event. (B) Characterization of U4.4 growth in serum-free suspension conditions (n = 3). (C) Example of U4.4 cell morphology in EX-CELL in passage 2 and (D) passage 7 compared to (E) the morphology of freshly detached U4.4 cells cultivated in an adherent flask with L-15 medium. Error bars indicate the standard error of the mean.

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