Fig. 6

Sequence analysis of OsRBSq3. (A) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii, Brachypodium distachyon, Hordeum vulgare, Lolium perenne, Oryza sativa ssp. japonica, Triticum aestivum, and Triticum Urartu. Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/) (B) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/) (C) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.