Fig. 2
From: Plasmonic imaging of living pancreatic beta-cell networks
SPRM reveals correlated oscillations in pancreatic beta-cells. (a) Brightfield image of MIN6 cells cultured on PLL-modified Au thin film. (b) Corresponding SPRM image with five regions of interest highlighting cells (1, 2 and 3) and the extracellular background (4 and 5). (c) Time-resolved reflectivity recorded over 130 s in HBSS with 10 mM glucose for the five regions shown in (b), inset shows a magnified view of a selected time window, indicated by t to t’, for the three cells which shows synchronised intensity oscillations. Traces appear synchronised but the background ROIs are anticorrelated. (d) Heat map displaying the correlation between signals extracted from ROIs 1 – 5 investigating signaling at the cellular ROIs (1–3) and the background ROIs (4, 5), where cells are not present.
