Fig. 3
From: Plasmonic imaging of living pancreatic beta-cell networks
Intracellular calcium oscillations in pancreatic beta cells within HBSS supplemented with 10 mM glucose. (a) Fluorescence image of MIN6 cells loaded with FLUO-4, excited at 450–490 nm, with 0–50 regions of interest (ROIs) indicated (0 denotes the background). Average fluorescence image of 3,000 frames of a field of MIN6 cells, with each measured cell labelled to indicate its 2D geography. (b) Cross-correlation matrix showing the Pearson correlation coefficients (threshold of 0.3) calculated between ROIs 0–50. (c) Exemplary 100-s traces of selected ROIs (band-pass filtered between 0.1 and 5 Hz), including clusters with high correlation {C8, C9}, {C11– C15}, {C16–C21}, {C23, C24}, {C44, C45}, alongside other ROIs selected at random.
