Fig. 5
From: Plasmonic imaging of living pancreatic beta-cell networks
Synchronised network oscillations are suppressed in the presence of a calcium channel blocker. (a) Brightfield image of MIN6 cells cultured on PLL-modified Au thin film. Scale bar 10 μm. (b) Corresponding SPRM image with 6 cellular regions of interest. (c) Time-series recordings from the six cellular ROIs presenting time-resolved reflectivity, under treatment with: 1) Hanks balanced salt solution (HBSS) without glucose; 2) HBSS supplemented with 10 mM glucose; and 3) HBSS supplemented with 10 mM glucose and 40 µM nifedipine. (d) Comparison of the effect of the three treatments on cells displaying the average amplitude profiles of the cells. Prior to identifying the amplitude profile, each signal was filtered between 0.1–15 Hz (see Methods) before standardization using the standard deviation over all the three recordings. Pairwise comparisons were performed using paired t‑tests, with p‑values adjusted for multiple comparisons (n = 6 cells) using the Bonferroni correction. Whiskers extend to 1.5 times the interquartile range (IQR).
