Fig. 8
From: Plasmonic imaging of living pancreatic beta-cell networks
Network analysis. (a, b) Brightfield and SPRM images of MIN6 cells, respectively. Scale bar 10 μm. (c) Connectivity matrices for the following conditions: baseline HBSS (c.i), HBSS supplemented with 10 mM glucose (c.ii) HBSS supplemented with 10 mM glucose and 40 μM nifedipine (c.iii). (d) Corresponding directed graphs represent cells ROIs as nodes, with edges (i.e. arrows) indicating patterns of directional connectivity and their associated weights. (e) Panels e(i) to e(iii) show examples of time series and their associated amplitude envelopes. Time-resolved connectivity, is presented for each of the above experimental conditions, measured via phase locking factor (PLF) and compared to amplitude correlation coefficient (ACC). PLF was calculated for a 10-s window with one second overlaps, for all cells and for each treatment. Similarly, ACC was computed by obtaining undirected correlation between the amplitude envelopes. (f) Boxplots depicting the mean undirected PLF (i) and the mean ACC (ii), calculated from their respective dynamic observations and averaged across six cells, are presented in panels e(i) and e(ii). In each boxplot, horizontal lines indicate the median values, while the boxes represent the IQR. Whiskers extend to 1.5 times the IQR. Paired t-tests were performed for multiple comparisons, with all P-values adjusted using the Bonferroni correction.
