Table 2 Sensitivity results of the quadruplex qPCR.

From: Development and validation of a quadruplex qPCR assay for simultaneous detection of GPV, GoCV, TMUV, and GAstV-Ⅱ in geese

Pathogens

Concentration

Total

Positive

Detection Rate

90% Detection Rate

GPV

1000 copies/µL

10

10

100%

>90%

100 copies/µL

10

10

100%

>90%

10 copies/µL

10

5

50%

<90%

GoCV

1000 copies/µL

10

10

100%

>90%

100 copies/µL

10

10

100%

>90%

10 copies/µL

10

6

60%

<90%

TMUV

1000 copies/µL

10

10

100%

>90%

100 copies/µL

10

10

100%

>90%

10 copies/µL

10

9

90%

= 90%

GAstV-Ⅱ

1000 copies/µL

10

10

100%

>90%

100 copies/µL

10

10

100%

>90%

10 copies/µL

10

7

70%

<90%

  1. Negative was determined by Cq > 35 and positive was validated by Cg < 35.
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