Table 3 Repeatability results (CT value) of the quadruplex qPCR.

From: Development and validation of a quadruplex qPCR assay for simultaneous detection of GPV, GoCV, TMUV, and GAstV-Ⅱ in geese

Plasmid Standard

Concen.

of template/(copies/µL)

Intra-coefficient of variation

Inter-coefficient of variation

Average ± SD

CV/%

Average ± SD

CV/%

GPV

108

12.04 ± 0.19

1.63

12.98 ± 0.08

0.60

107

15.74 ± 0.09

0.55

16.38 ± 0.30

1.82

106

18.52 ± 0.23

1.25

19.76 ± 0.40

2.02

105

21.87 ± 0.13

0.57

23.27 ± 0.15

0.69

104

25.89 ± 0.11

0.41

26.36 ± 0.34

1.28

103

28.64 ± 0.12

0.43

29.76 ± 0.30

1.00

102

32.29 ± 0.30

0.92

31.82 ± 0.30

0.94

TMUV

108

11.52 ± 0.05

0.46

11.32 ± 0.11

0.97

107

15.23 ± 0.11

0.75

14.78 ± 0.17

1.13

106

18.79 ± 0.21

1.14

18.27 ± 0.27

1.43

105

22.36 ± 0.01

0.03

21.85 ± 0.22

1.01

104

25.61 ± 0.16

0.61

25.11 ± 0.21

0.85

103

28.19 ± 0.24

0.84

28.69 ± 0.15

0.52

102

32.01 ± 0.84

2.62

32.06 ± 0.09

0.28

GAstV-Ⅱ

108

11.95 ± 0.07

0.58

12.67 ± 0.03

0.25

107

15.68 ± 0.01

0.06

16.38 ± 0.04

0.21

106

19.06 ± 0.02

0.09

19.67 ± 0.02

0.12

105

22.75 ± 0.02

0.06

23.28 ± 0.03

0.11

104

26.06 ± 0.09

0.33

26.69 ± 0.15

0.56

103

29.13 ± 0.10

0.34

29.60 ± 0.27

0.92

102

32.88 ± 0.30

0.79

32.23 ± 0.17

0.53

GoCV

108

11.81 ± 0.05

0.43

11.54 ± 0.10

0.86

107

14.82 ± 0.49

3.30

14.41 ± 0.26

1.79

106

18.11 ± 0.23

1.29

17.78 ± 0.23

1.27

105

21.75 ± 0.18

0.85

21.17 ± 0.15

0.72

104

24.96 ± 0.08

0.32

24.56 ± 0.17

0.69

103

28.04 ± 0.09

0.33

27.19 ± 0.08

0.26

102

31.74 ± 0.67

2.09

30.82 ± 0.39

1.27

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