Table 3 FTIR characteristic peaks for chitosan, methacrylated chitosan, Eosin Y, triethanolamine, and dithiothreitol.

From: A cell-laden methacrylated chitosan-based photocrosslinkable hydrogel for bone tissue engineering and its in vitro structural and biological characterization

Material

Characteristic peaks and their wave number (cm− 1)

Chitosan (Cs)

νas(C–O–C), 1150 cm− 1 69 – ν(C–N) for amide III, ~ 1317 cm− 1 69 – ν(C = O) for amide I, 1650–1655 cm− 1 69,70 – δ(N–H) for amide II in the N-acetyl-D-glucosamine (GlcNAc) units, ~ 1555–1560 cm− 1 71,72 – CO–NH for amide III, 1320 cm− 1 70

Methacrylated chitosan (MECs)

C = CH2, 804 cm− 1 11 – ν(C–N) for amide I, 1625 cm− 1 11 – CH3 1404–1409 cm− 1 71 – CONH, 3100 cm− 1 11 – ester bond, ~1710 cm− 1 11

Eosin Y (EY)

ν(C–C), 1260 cm− 1 73 – ν(C–O–C), 1215,1330 cm− 1 73 – ν(C–C), 1317–1348 cm− 1 74 – ν(C–O), 1200–1260 cm− 1 75 – ν(C–C), νs(COO−), ~1395 cm− 1 73 – ν(C–C), νs(COO−), ~1460 cm− 1 73 – lactone conformation or ν(C = O), ~1750 cm− 1 76 – νs (C–H) ~ 2848 cm− 1 77 – νas (C–H), 2922 cm− 1 77

Triethanolamine (TEOA)

ν(C–N), ~1450 cm− 1 77 – δ(–CH2–), ~1468 cm− 1 78– ν (–CH2–), 2845–2925 cm− 1 78– ν (C–H), ~2950 cm− 1 77

Dithiothreitol (DTT)

–SH, 2550–2600 cm− 1 79 – ν(monomers), ~2545 cm− 1 80 – ν(dimmers), ~2564 cm− 1 81 –R1–S–S–R2–, 730–750 cm− 1 82 – R1–S–R2, 760–785 cm− 1 82

  1. ν: stretching vibration – δ: bending vibration – as: asymmetric – s: symetric.