Fig. 1
Effect of targeted deletion of hepatic circadian clock genes on liver sysRAAS components. Relative expression of (a) Agt, (b) Enpep, (c) Lnpep, (d) Bmal1, (e) Per1, and (f) Per2 in LCre (black solid lines), LBmal1 (green dotted lines) and LPer1/2 (magenta dashed lines) mice at six different zeitgeber time points with 4-hour intervals. Green stars indicate significant differences between LCre and LBmal1, and magenta stars indicate significant differences between LCre and LPer1/2. Data are presented as mean ± SEM (n = 3). (g) western blot analysis of the indicated proteins at ZT8 and quantification (n = 4) (h-j). On the left are the positions of size markers [kDa]. Agt, AGT: Angiotensinogen; Enpep: Glutamyl aminopeptidase; Lnpep: Leucyl and Cystinyl aminopeptidase; Bmal1, BMAL1: Brain and muscle ARNT-like protein 1; Per1: Period 1; Per2, PER2: Period 2; HSP90: heat shock protein 90. Two-way ANOVA with Tukey’s multiple comparisons test, * p < 0.05; ** p < 0.01; *** p < 0.001.
