Fig. 4

Effect of targeted deletion of hepatic circadian clock genes on plasma sysRAAS components. (a) AGT, (b) renin, (c) ACE, and (d) aldosterone in LCre (black solid lines), LBmal1 (green dotted lines) and LPer1/2 (magenta dashed lines) mice at six different zeitgeber time points with 4-hour intervals. Green stars indicate significant differences between LCre and LBmal1, and magenta stars indicate significant differences between LCre and LPer1/2. Data are presented as mean ± SEM (n = 3). AGT: Angiotensinogen. ACE: Angiotensin I-converting enzyme. Two-way ANOVA with Tukey’s post hoc test, * p < 0.05; ** p < 0.01; *** p < 0.001.