Fig. 5 | Scientific Reports

Fig. 5

From: Anti-PD-1 immune checkpoint inhibitor-induced cardiotoxicity is associated with dysfunctional metabolism, muscle wasting and autophagy

Fig. 5

Autophagic flux analysis in cardiac muscle via Fluorescent Microscopy. Yellow puncta (b) represent early-phase autophagosomes. Red puncta (c) represent late-phase autolysosomes. Effects of ICI treatment on protein levels of p62 (d) in cardiac muscle. Representative fluorescent images have been taken at 40x magnification (a). Yellow arrows point towards yellow puncta/early-phase autophagosomes, red arrows point towards red puncta/late-phase autolysosomes (a). Cropped, representative western blot images (d); original blots are presented in supplementary files. CON, control group (n = 5); ICI, ICI treated group (n = 5); N = 10. Values are reported as mean ± SD. *Significant difference, P < 0.05. **Significant difference, P < 0.01.

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