Fig. 1 | Scientific Reports

Fig. 1

From: Exploiting attP landing sites and gypsy retrovirus insulators to identify and study viral suppressors of RNA silencing

Fig. 1The alternative text for this image may have been generated using AI.

A transgenic reporter system designed to silence the D. melanogaster white gene using a dsRNA hairpin sequence. In the sensor lines, both the white inverted repeat sequence and the yeast GAL4 protein are regulated by the GMR enhancer, which is active in immature and adult retinal tissue where the endogenous white gene is also expressed. The tissue-specific expression of the inverted repeats produces hairpin-loop RNA, inducing RNAi that targets the D. melanogaster white gene. In the test lines, VSR proteins or controls (such as EGFP) are under the transcriptional control of a D. melanogaster promoter linked to GAL4-responsive upstream activating sequence (UAS) repeats. When the test lines are crossed with reporter lines, GAL4 drives the tissue-specific expression of VSR or control sequences. Additionally, some test lines were created with gypsy insulator sequences positioned either upstream, downstream, or flanking the GAL4-responsive elements.

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