Fig. 3 | Scientific Reports

Fig. 3

From: Exploiting attP landing sites and gypsy retrovirus insulators to identify and study viral suppressors of RNA silencing

Fig. 3The alternative text for this image may have been generated using AI.

Suppression of GMR-hairpin mediated silencing of white by DCV-1A, CrPV-1A, or FHV-B2. (A) Far left, (1) Eye pigmentation of GMR-GAL4; UAS-GFP flies (VK1). (2) Eye color observed in flies with GMR-whiteIR; GMR-GAL4; UAS-GFP transgenes (VK1). (3) Suppression of white silencing mediated by DCV-1A expressed from the VK1 landing site with flanking gypsy insulators. (4–5) Lack of white silencing inhibition by FHV-B2 expressed from the VK1 or attP18 landing sites with flanking gypsy insulators. (6) Suppression of white silencing by CrPV-1A expressed from the attP18 landing site with flanking gypsy insulators. Eye pigment levels were measured in three separate experiments using age-matched 4-day-old adults, and are expressed as a percentage relative to the pigment levels measured in the GMR-GAL4; UAS-GFP (VK1) controls. Error bars represent standard error. Significance was calculated by Student’s t-test.

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