Fig. 6 | Scientific Reports

Fig. 6

From: A novel variant p.Y250C of FZD4 influences Norrine/β-catenin signaling pathway that associates with familial exudative vitreoretinopathy (FEVR)

Fig. 6The alternative text for this image may have been generated using AI.

Subcellular localizations and nuclear entry ratios of wild-type and mutant FZD4 proteins. (A–C) Subcellular localization of wild-type FZD4 protein express throughout the cytoplasm in HeLa cells, without highlights. (D–F) Subcellular localization of wild-type FZD4 protein aggregate on cell membrane and cytoplasm of HeLa cells, with highlights. (G–I) Subcellular localization of mutant FZD4 protein express throughout the cytoplasm in HeLa cells, without highlights. (J–L) Subcellular localization of mutant FZD4 protein aggregate on nucleic membrane and nucleus of HeLa cells, with highlights. The highlights represent the aggregation of GFP-labeled FZD4 protein. FZD4 protein shows in the green image on the left side of the panel; nucleus shows in the blue image in the middle; the merge of them shows on the right side. M: The ratio of wild-type FZD4 and mutant FZD4 proteins entering the nucleus. FZD4-WT represents the proportion of wild-type FZD4 protein entering the nucleus, while FZD4-MUT denotes the proportion of mutant FZD4 protein entering the nucleus. *P < 0.05. Scale bar: 5 μm. Magnification: × 50.

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