Fig. 4
From: Neonatal hyperoxia exposure causes cerebellar lesions and behavioral abnormalities in rats
Neonatal hyperoxia exposure affected migration of granular cells in the external granular layer. (A) Representative photomicrographs of the external granular layer in the P7 cerebellar tissue section (Bar = 500 μm). The right panel shows a higher-magnification view. The black arrow indicates the magnified area. The fluorescent blue line surrounds the external granular layer. (B) Ratio of the external granular layer volume to the total cerebellar hemisphere volume at P7 was significantly higher in the hyperoxia group than in the control group (control, n = 8; hyperoxia, n = 7). (C) Representative photomicrographs of the P10 cerebellar tissue section showing BrdU-positive cells (Bar = 50 μm; Insets show higher-magnification views [bar = 5 μm]; Black arrow indicates the area magnified). (D) The number of BrdU-positive cells in the inner margin of the external granular layer at P10 was significantly higher in the hyperoxia group than in the control group (control, n = 9; hyperoxia, n = 6). (E) The number of BrdU-positive cells in the internal granular layer at P10 was not significantly different between the control and hyperoxia groups (control, n = 9; hyperoxia, n = 6). Data represent mean ± standard deviation; *p < 0.05 and **p < 0.01.
