Fig. 6

Effect of eugenol on the brain histopathological findings in ACR-induced brain toxicity in rats. Photomicrograph showing the brains of (a, b, and c) the control group, showing normal cerebral cortex with normal round neurons (black arrows) (a), normal hippocampal neurons (black arrows) (b), and normal Purkinje cells (black arrows) at the interface of the outer molecular and inner granular cell layers (c), (d, e, f) ACR group showing an increased number of degenerated neurons with intensely eosinophilic cytoplasm and pyknotic nuclei in the cerebral cortex (black arrows) (d), neuronal degeneration with neuronophagia in the hippocampus (black arrows) (e), and degeneration of the Purkinje cells (black arrows), associated with proliferation of astrocytes (red arrows) (f), (g, h, i) Eu50 + ACR group showing a significant reduction in the number of degenerating neurons in the cerebral cortex (black arrows) (g) and hippocampus (black arrows) (g), in addition to a decreased number of degenerate Purkinje cells (black arrows) (i) and (j, k, l) Eu100 + ACR group showing sparse degenerate neurons in the cerebral cortex (black arrow) (j) and hippocampus (black arrow) (k) and normal Purkinje cells (black arrows) (l). (Stain: H&E, scale bar = 100 µm).