Fig. 4
From: Modeling fatty liver disease and progression with stem cell derived hepatocytes
Raman spectroscopy and imaging enable label-free identification of lipid uptake in HLCs. (a) SRS images of HA at the 2848 cm− 1 symmetric carbon-hydrogen stretching modes of lipids. (1) Control, (2) OA-treated, and (3) PA-treated. Representative results of 3 biological replicates of differentiation, where a.u. is for the arbitrary units. (b) Raman spectra of the C-H stretch region of HA from control, OA-treated, and PA-treated. Representative results of 3 biological replicates of differentiation, where n = 3 measured HA per condition. (c) Raman signal strength of the 3015 cm− 1 vibration of unsaturated lipids. Representative results of three biological replicates of differentiation, and n = 5 measured HA per condition. The p-values of the Shapiro-Wilk test are 0.5312, 0.4931, and 0.3253 for Ctrl, OA, and PA, respectively. The p-value is less than 0.0001 for Ctrl vs. OA and OA vs. PA, and 0.5024 for Ctrl vs. PA.
