Fig. 2 | Scientific Reports

Fig. 2

From: Microscopy and spatial-metabolomics identify tissue-specific metabolic pathways uncovering salinity and drought tolerance mechanisms in Avicennia marina and Phoenix dactylifera roots

Fig. 2

Mangrove seedlings and triple staining of cell wall components of mangrove roots cross-sections. (A) Different stages of mangrove seeds germination. (B-D’’’) Triple staining of meristematic cross-sections. (B-B’’’) Full cross-section 10x magnification. Merged (B), Basic Fuchsin (B’), Fluorol Yellow (B’’) and Calcofluor White (B’’’). (C-C’’’) Epidermis and exodermis. Merged (C), Basic Fuchsin (C’), Fluorol Yellow (C’’) and Calcofluor White (C’’’). (D-D’’’) Endodermis and vasculature. Merged (D), Basic Fuchsin (D’), Fluorol Yellow (D’’) and Calcofluor White (D’’’). (E-G’’’) Triple staining of cross-sections from the differentiation zone. (E-E’’’) Full cross-section 10x magnification. Merged (E), Basic Fuchsin (E’), Fluorol Yellow (E’’) and Calcofluor White (E’’’). (F-F’’’) Epidermis and exodermis. Merged (F), Basic Fuchsin (F’), Fluorol Yellow (F’’) and Calcofluor White (F’’’). (G-G’’’) Endodermis and vasculature. Merged (G), Basic Fuchsin (G’), Fluorol Yellow (F’’) and Calcofluor White (F’’’). Scale bars: 2 cm (A), 869.5 μm (B-B’’’), 36.8 μm (C-C’’’), 49.1 μm (D-D’’’, F-F’’’, G-G’’’), 867.5 μm (E-E’’’). Co: Cortex; CS: Casparian Strips; diff: differentiation; En: Endodermis; Ep: Epidermis; Ex: Exodermis; me: meristem; Pi: Pith; Xy: Xylem.

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