Fig. 13 | Scientific Reports

Fig. 13

From: Integrating single-cell RNA-Seq and bulk RNA-Seq data to explore the key role of fatty acid metabolism in hepatocellular carcinoma

Fig. 13

Validation of PTGES3 Expression Levels and the Significant Inhibition of Proliferation, Invasion, and Migration in Liver Cancer Cells following PTGES3 Gene Knockout. (A) Assessment of PTGES3 mRNA expression in various liver cancer cell lines. (B-C) Evaluation of the knockout efficiency of PTGES3 mRNA in HCCLM3 and Huh-7 cells. (D-E) The CCK-8 assay reveals that the knockout of the PTGES3 gene significantly inhibits the proliferative capacity of HCCLM3 and Huh-7 cells. (F) Immunohistochemistry (IHC) experiments confirm elevated PTGES3 expression in liver cancer tissues, with correspondingly higher IHC scores. (G-H) Knockout of the PTGES3 gene significantly reduces the migratory and invasive capabilities of HCCLM3 and Huh-7 cells. (I) Statistical representation of the experimental data for HCCLM3 and Huh-7 cells; * p < 0.05; ** p < 0.01; *** p < 0.001; NS: not statistically significant.

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