Fig. 2

Analysis of single-cell RNA sequencing data in hepatocellular carcinoma. (A) UMAP representations of nine distinct cell types, processed through filtration, normalization, and analysis with the Seurat package. (B) Bubble plots with annotations of cell type-specific markers, utilizing the SingeR package for annotation. (C-D) Utilization of the ggplot2 package to depict the distribution of cell types across various tissues and patient stages. (E) Bubble plots evaluating the enrichment of metabolic pathways, employing the scMetabolism package for analysis.