Fig. 1 | Scientific Reports

Fig. 1

From: Nano-scaffold containing functional motif of stromal cell-derived factor 1 enhances neural stem cell behavior and synaptogenesis in traumatic brain injury

Fig. 1

(A) Prediction of β-sheet structure and solvent accessibility of Nano-SDF. (B) Visualization of the 3D β-sheet structure and solvent accessibility of Nano-SDF. (C) Representative GC-MS data for the nanofiber (Nano-SDF). Key mass/charge (m/z) peaks at 1060.05 [M + 4 H] + 4 and 1413.10 [M + 3 H] + 3, confirming the molecular identity of the nanofibers. Additional peaks correspond to various molecular fragments. The molecular weight of the nanofibers was estimated to be ~ 4235.74 g/mol, aligning with the expected molecular weight of the scaffold. (D) A representative HPLC chromatogram was obtained for the nanofiber (Nano-SDF). Two major peaks with retention times of 11.693 and 12.257 min, indicating a purity of over 99%. The analysis confirms the high purity and stability of the synthesized nanofibers within a 25-minute assessment window. (E) SDF-1 nanofibers formed a gelatinous material after the addition of an ion solution (PBS).

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