Fig. 5 | Scientific Reports

Fig. 5

From: Exploring the protective role of caffeine against Taraxacum-Induced ribotoxic stress mediated through autophagy and mitochondrial depolarization

Fig. 5

The rescue effects of caffeine on the T. formosanum-induced 18S and 28S rRNAs cleavage in HeLa cells. (A-D) HeLa cells were treated with 7 mg/mL T. formosanum for 16 h alone or in the presence of pretreated with indicated amounts of caffeine for 2 h, after which their cell lysates were subjected to (A) total RNA microcapillary electrophoresis analysis and microcapillary electropherograms were generated by Agilent 2100 bioanalyzer. (B) total RNA agarose gel electrophoresis analysis; (C) RT-PCR analysis using primer pairs against the indicated mRNAs, with GAPDH mRNA as the mRNA loading control and 18S rRNA as an internal control; (D) western blot analysis using antibodies against the indicated proteins, with ACTN as the protein loading control. M, bp: the molecular weight of rRNA. M, kDa: the molecular weight of protein.

Back to article page