Fig. 7 | Scientific Reports

Fig. 7

From: Exploring the protective role of caffeine against Taraxacum-Induced ribotoxic stress mediated through autophagy and mitochondrial depolarization

Fig. 7

The rescue effects of caffeine on the T. formosanum-dependent mRNAs and proteins in HeLa cells. (A-C) HeLa cells were treated with 7 mg/mL T. formosanum for 16 h alone or in the presence of pretreated with indicated amounts of caffeine for 2 h, after which their cell lysates were subjected to (A) total RNA agarose gel electrophoresis analysis; (B) RT-PCR analysis using primer pairs against the indicated mRNAs, with 18S rRNA as an internal control; (C) western blot analysis using antibodies against the indicated proteins, with ACTN as the protein loading control. The red single-arrow is the cleaved 28S rRNA and the double-arrow is the cleaved 18S rRNA. M, bp: the molecular weight of rRNA. M, kDa: the molecular weight of protein.

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