Fig. 2
From: 20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells
Ang II stimulates 20-HETE production and enhances CYP4A1 expression in H9c2 cells. (A) ELISA was used to measure 20-HETE content in H9c2 cells treated with varying doses of Ang II (0.01–2 µmol/L) for 24 h. *P < 0.05 vs. Control group, n = 3. (B) Effects of Losartan and PD123319 pretreatment on the Ang II-induced 20-HETE production in H9c2 cells. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II group, n = 3. (C) RT-qPCR analysis of the expression of CYP4A1 with different treatments. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II group, n = 5. (D) Western blot analysis of the protein levels of CYP4A in H9c2 cells with different treatments. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II group, n = 3. (E) AT1 receptor mRNA expression levels in the different groups were quantified. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (F) Representative blots and quantification of AT1 receptor protein expression. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3.
