Fig. 3
From: 20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells
Inhibition of 20-HETE production or blockade of its action attenuates Ang II-induced intracellular ROS accumulation in H9c2 cells. (A) Representative images of the levels of ROS generation as measured by DHE staining (scale bar: 20 μm). (B) Analysis of mean fluorescence intensity in each group. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (C–D) Western blot analysis and quantification of the expression of NOX2 and p-p47phox in the indicated groups. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (E) NADPH oxidase activity was measured by cytochrome c reduction method. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 5.
