Fig. 4
From: 20-HETE mediates Ang II-induced cardiac hypertrophy via ROS and Ca2+ signaling in H9c2 cells
Inhibition of 20-HETE production or blockade of its action reduces Ang II-induced mt-ROS generation and oxidative stress in mitochondria. (A) Representative immunofluorescence images of MitoSOX Red (red) and DAPI (blue) staining (scale bar: 20 μm). (B) Representative images of 8-OHdG (green) and DAPI (blue) stained H9c2 cells. (scale bar: 20 μm). (C) Quantification of mean fluorescence intensity of mt-ROS generation in each group. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (D) Quantification of mean fluorescence intensity of 8-OHdG expression in each group. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (E) Representative blots and quantification of NOX4 protein expression. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 3. (F) Activity of Mn-SOD was measured by WST-8 colorimetric assay. *P < 0.05 vs. Control group, #P < 0.05 vs. Ang II or 20-HETE group, n = 5.
