Fig. 3
From: B cells enhance IL-1 beta driven invasiveness in triple negative breast cancer
(a) Wound healing assay of SUM159 and MCF12A cells following 72-h co-culture with B lymphocytes. Results were calculated as mean +/− standard deviation of the distance between the leading edges of the wound at 0 and at 12 h. (b) In vitro Matrigel invasion assay using TNBC cell lines cultured cells with and without B cells and IL-1RA treatment. Representative 10× images of randomly selected fields show invaded cells stained with Crystal Violet. (c) Zymography analysis of MMP2 and MMP9 in SUM159 and MCF12A cell supernatants following 72-h co-culture with EBV B cells. Upregulation of MMP activity in zymogram assays is blocked in SUM159 (d) and MCF12A (e) cells co-cultured for 72 h with EBV B cells in cells co-treated with IL-1RA. Graphs show the mean of at least three independent experiments. Error bars represent standard deviations Significance is indicated by asterisk, *p < 0.05, **p < 0.01, ***p < 0.001.
