Fig. 2
Fragment counts per million mapped reads (FPM) in the methanogenesis pathway of M. barkeri. For each transcriptomic library, FPM was calculated using the robust median ratio method to account for sequencing differences in library sizes and RNA compositions in samples. (A) FPM from 80:20 H2:CO2-incubated experiments. (B) FPM from Mars gas mix-incubated experiments. Genes are ordered spectrally in the legend from red to violet according to relative steps in the hydrogenotrophic methanogenesis pathway (i.e., red, orange = early pathway steps; yellow, green = mid-pathway steps; blue, violet = terminal steps). Note the difference in scale of the y-axes between A and B.
