Fig. 4
Differential gene expression (log2-fold change) of the methanogenesis pathway in M. barkeri under Mars gas mix headspace relative to the 80:20 H2:CO2 headspace control. Text sizes of individual genes are scaled according to average normalized transcript copy numbers. Statistically significant differential expression is indicated by red genes and determined via Wald test (P ≤ 0.05). Related gene subunits are clustered into single enzymes (cytoplasmic = light blue; periplasmic = yellow). Metabolites and pathways directionality are indicated by serifed text and dark grey arrows, respectively. Full names of listed genes and metabolites are found in Tables S7 and S8, respectively. Differential expression graphs with respect to temperature and pressure variables are found in Figures S4 and S5, respectively.
