Fig. 1 | Scientific Reports

Fig. 1

From: Cell-free expression and SMA copolymer encapsulation of a functional receptor tyrosine kinase disease variant, FGFR3-TACC3

Fig. 1

Cell-free expression and purification of RT112 in SMALPs. (a) Fluorescence normalised against the maximum observed with time in hours. Error bars represent the standard error across 3 repeats. (b) Size-exclusion chromatogram of RT112. Black trace represents ligand-free protein, blue represents ligand-bound (in the presence of FGF1). Peaks 1, 2 and 3 eluted at approx. 2.20, 2.70 and 3.30 mL respectively. (c) PAGE analysis of fractions collected during size-exclusion chromatography of RT112. Fractions 1, 2 and 3 from b. were analysed by SDS and SMA-PAGE, and visualised using silver stain and in-gel GFP fluorescence. Denatured ladder (M) was obtained from the SDS-PAGE pre-silver-stain and aligned to SDS-PAGEs. Native ladder (M’) was obtained post silver stain and aligned to SMA-PAGEs.

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