Fig. 7
Cell death protein expression in differentiated colonoids in response to tofacitinib or budesonide pre-treatment with or without TNF + Poly(I:C). (a) Immunoblots showing pMLKL, MLKL total, caspase 3 and cleaved caspase 3 protein expression in colonoids from N = 6 donors (4HC and 2UC) cultured at 2% oxygen. Original full blot images are presented in the Supplementary Figure S2. (b, c) Quantification of pMLKL/MLKL or cleaved caspase 3/caspase 3 at group level in response to different treatments (1–6) (d, e) Paired quantification of pMLKL/MLKL or cleaved caspase 3/caspase 3 protein expression between selected treatments. The colonoids were pre-treated with tofacitinib or budesonide for 3 days alone or before TNF + Poly(I :C) (see Fig. 1). The x-axis shows the treatment, and the y-axis shows fold induction. Fold expression is generated by normalizing to vehicle control (0.033% DMSO) for each donor and further normalized to GAPDH expression. Differences were evaluated using paired t-test. *P < 0.05.
