Fig. 4

The box plot of ASD-related gene expression in NSCs. The expression levels of Atp1a3, Nefl, L1cam, Grin1, and Gabrb1 were determined in both sexes and separately in males and females. The qRT-PCR analyses revealed that Atp1a3, Grin1, and Gabrb1 were significantly upregulated in the NSCs of both sexes that were prenatally exposed to BPA. All genes except L1cam were significantly upregulated in the NSCs in the male group. The expression level of every gene tended to increase in the NSCs in the female group but was not statistically significant. * P-value < 0.05, paired t-test.