Fig. 5

Neurosphere assays. (a) NSCs of control and BPA treatment pups, both males and females, were cultured in vitro for 7 days and 14 days to form neurospheres. (b) Quantitative analysis of the mean size of neurospheres, both sexes, derived from control or BPA treatment. The mean size of neurospheres derived from BPA treatment was significantly greater than that of the control group. (c) Quantitative analysis of the mean size of neurospheres, separately male and female, derived from control or BPA treatment. The mean size of neurospheres derived from BPA treatment was significantly greater than that from the control group, both in males and females. ((a) Scale bar = 100µm)