Fig. 1 | Scientific Reports

Fig. 1

From: Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress

Fig. 1

Subcellular localization of exogenous-expressed LEA proteins in sheep fibroblasts. (a–c) pTag-WCOR410-RFP; (d–f) pTag-RAB17-GFP. (a) WCOR410-RFP fusion protein displays membrane localization (arrowheads), (b) nuclei stained with Hoechst 33342; (c) merged; (d) RAB17-GFP fusion protein displays cytoplasmic and nuclear localisation (arrowheads); (e) nuclei stained with Hoechst 33342; (f) merged. (g) Western blot verified expression of pTag-RAB17-GFP in the somatic cells; (h) Western blot verified expression of pTag-WCOR410-RFP in the somatic cells. Protein extract from non-transfected cells (mock) and transfected with empty vectors EV-GFP and EV-RFP was used as a control. Scale bars, 20 μm (a–f).

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