Fig. 4 | Scientific Reports

Fig. 4

From: Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress

Fig. 4

Differences in mitochondria and cytoskeleton cold stability. (a) Mitochondria staining performed using MitoTracker Green or MitoTracker Red dyes, revealing mitochondrial network morphology in cells cultured at 38.5 °C (CTR) and exposed to cold stress under different treatments (CTR, Vitamin E, WCOR410-RFP and RAB17-GFP). The nuclei were counterstained with Hoechst 33342. Scale bar, 20 μm. (b) Cytoskeleton staining performed using Phalloidin green to stain actin microfilaments (left panel) and tubulin immunofluorescence to stain microtubules (right panel) on cells cultured at 38.5 °C (CTR) and exposed to cold stress with different treatments (CTR, Vitamin E and WCOR410-RFP). Alpha-tubulin detected with Goat anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor 488. Nuclei were counterstained with Hoechst 33342. Scale bar, 20 μm.

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