Fig. 5 | Scientific Reports

Fig. 5

From: Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition

Fig. 5

Inhibition of MAPKs phosphorylation/activation by CSE in TNF-α/IFN-γ-stimulated HaCaT cells. (A) Typical Western blots showing the time course of MAPKs phosphorylation (p-JNK, p-ERK, and p-p38) in HaCaT cells during stimulation with TNF-α/IFN-γ (10 ng/mL each) alone and following pretreatment with CSE. (B) Treatment with TNF-α/IFN-γ induced a transient rise in p-JNK, p-ERK, and p-p38 expression levels that peaked at around 30 min. These responses were suppressed dose-dependently by 1 h of CSE pretreatment. (C) Bar graphs of p-JNK, p-ERK and p-p38 band densities relative to total form band density (the gel loading control). All values are the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. TNF-α/IFN-γ-stimulated group; #p < 0.05 vs. control group. Dexa: dexamethasone (positive control).

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