Table 4 Separation conditions of UPLC in CSE.
Item | Conditions | |||||
|---|---|---|---|---|---|---|
Column | Phenomenex Kinetex C18, 5 μm, 250 × 4.6 mm | |||||
Mobile phase | Time | 2 min | 40 min | 42 min | 44 ~ 47 min | |
A | 0.2% H3PO4 in water | 85% | 70% | 0% | 85% | |
B | Acetonitrile | 15% | 30% | 100% | 15% | |
Flow rate | 1.0 mL/min | |||||
Column oven temperature. | 40 °C | |||||
Detector | UV 240 nm | |||||
Retention time | 42 min | |||||