Fig. 4
From: Dynamic culture system advances the applications of breast cancer organoids for precision medicine
Fluid shear stress alters the morphological characteristics and gene expression of breast cancer organoids. (a) Extended organoids culture time in Flow group from 15 days to 30 days. (b) Representative microscopic images of long-term cultured organoids in Flow group. Scale bar: 200 μm. (c) Histological and immunohistochemical images showing the structural organization and status of breast cancer-related markers (Ki67, CK7, E-cadherin, GATA3, Her-2, ER, PR) in primary tumors and organoids cultured in Flow group for 30 days. Scale bar: 50 μm. (d) Representative HE image of BC-3 organoids cultured in Flow group for 30 d, detachment of cells and chromatin marginalization in the center of organoids. Scale bar: 50 μm.(e) Representative HE and IHC(ki67) image of BC-3 organoids cultured in Flow group for 30 d, Some organoids began to form concentric circle structures. Scale bar: 50 μm.
