Fig. 8
Three pairs of cell lines (C2 vs. DS2, C4 vs. DS3, and C5 vs. DS4) were used for downstream analyses, including RT-qPCR, and western blot. All measurements were normalised by dividing DS values with their corresponding controls. (A) Western blot for the total REST, DCX and NFIA protein in DS-derived NPCs and controls; (B-G) Protein relative quantitative analysis and RT-qPCR for REST, DCX and NFIA protein and mRNA expressed in DS-derived NPCs and controls; (H, I) RT-qPCR for detection of JAK2 and STAT3 mRNA expressed in DS-derived NPCs and controls, respectively. *P < 0.05, **P < 0.01, *** P < 0.001. Con and C, Control; DS, Down syndrome.
