Fig. 1

DRAM1 was upregulated in gastric cancer (GC) and was associated with the progression of GC. (a) DRAM1 mRNA was significantly higher in GC tissues than the adjacent normal tissues (***: P = 0.001) based on the TCGA dataset (375 GC tissues and 32 adjacent normal tissues). (b) Survival analysis based on the TCGA dataset indicated that higher DRAM1 expression was associated with poor OS in patients with GC (P = 0.039). (c) DRAM1 protein expression was confirmed in four pairs of GC tissues by western blotting. (d) Bar chart was the quantitative analysis of DRAM1 protein in four pairs of GC tissues (***: P < 0.001;**: P = 0.003). (e) Western blot was used to confirm the expression of DRAM1 in gastric cancer cell lines. (f) The histogram showed the expression of DRAM1 mRNA in gastric cancer cells . Compared with normal gastric mucosa, DRAM1 was low expressed in HGC27 cells (**: P = 0.005) and high expression in AGS, MKN45, MGC803, and SGC7901 cells (****: P < 0.001). (g) Bar chart represented the efficiency of DRAM1 knockdown in AGS cells (Ctrl vs shDRAM1#1, **: P = 0.008; Ctrl vs shDRAM1#2, **: P = 0.008). (h) Bar chart represented the efficiency of overexpression of DRAM1 in HGC27 cells (NC vs OE, ***: P < 0.001).