Table 2 Summary of three HBV WGS pipelines, comparing time, cost and outputs.
Capture—Illumina | HEP-TILE—Nanopore | HEP-TILE—Illumina | |
|---|---|---|---|
Time required for enrichment and library prep | 1.5 days (capture overnight) | 1.5 days (PCR overnight) | 1.5 days (PCR overnight) |
Sensitivity (HBV DNA VL) for generating HBV WGS | > 5.5 log10 IU/ml | > 1.5 log10 IU/ml | > 1.5 log10 IU/ml |
Consensus sequence accuracy* | 100% | 100% | 99.98% |
Cost per sample** (enrichment & library prep reagents) | £29 | £17 | £25 |
Cost per sample*** (sequencing) | £25 E.g.multiplexed on partial lane on Novaseq X, 1 million reads per sample | £5.60 E.g. 96 samples multiplexed per flow cell, bulk purchasing 12 flow cells, 100,000 reads per sample | £2.50 E.g. multiplexed on partial lane on Novaseq X, 100,000 reads per sample |
Throughput | Platform dependent e.g. Miseq vs. Novaseq X | Platform dependent e.g. Minion vs. Promethion | Platform dependent e.g. Miseq vs. Novaseq X |
Portability | No | Yes with MinION | No |
