Fig. 3 | Scientific Reports

Fig. 3

From: Identification of an additional periplanone receptor family gene preferentially expressed in the male antennae of the American cockroach

Fig. 3

FISH to discriminatory stain PameOR1 and PameOR1-like. (A) Nucleotide sequence alignment of the 3’ region of the protein-coding sequence and the 3’ UTR region of PameOR1 and PameOR1-like, used as templates for riboprobe synthesis. The letters are shaded according to alignment strength. The protein-coding sequence is outlined with a red line. (B) Dot plot representation of nucleotide sequence identity between the riboprobe region ofPameOR1 and PameOR1-like. (C) Single-color FISH for the discriminatory detection of PameOR1 and PameOR1-like mRNAs in the male adult antennae. As a positive control, we used the riboprobe from our previou study11, which simultaneously detects both PameOR1 and PameOR1-like mRNAs. OSNs located beneath the cuticle were stained (arrowheads). Scale bars = 10 μm. (D) Magnified views of a single sw-B sensilla. In individual sw-B sensilla, a single OSN expressing either PameOR1 or PameOR1-like was detected, respectively (arrowheads). Scale bars = 5 μm.

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