Fig. 4
Pde4b gene knockout inhibits the progression of IDD lesions in mice model. (A) Representative magnetic resonance image of PDE4B KO and WT mice before and after modeling. The white arrow indicates the needle puncture site. (B) Representative pathological images of intervertebral disc tissue from PDE4B KO and WT mice stained with safranin and fast green and immunohistochemically detected phosphorylated JNK. (C) Typical MRI schematic diagram, histological degeneration score and quantification of p-JNK positive cells in NP tissues. Statistical differences between groups were analyzed by two-way ANOVA. The data shown are the means ± SEMs. The biological replicates are 3–5. * p < 0.05, ** p < 0.01, ***p < 0.001.
