Fig. 2
A. baumannii transcriptional analysis in response to NO. (A) Functional classification of NO-responsive genes resulting from RNA-seq analysis. White and black bars indicate the number of genes with decreased and increased expression, respectively, in cells treated with 50 µM of Spermine NONOate for 15 min. (B) RNA-seq analysis. Statistically significant (FDR < 0.05) upregulated and downregulated genes are depicted in green and red, respectively. Horizontal dashed lines are placed at logFC = -1 and logFC = 1. The vertical dashed line is placed at average log counts per million = 3. (C-D) Quantitative real-time PCR (qRT-PCR) of selected upregulated genes (C) or downregulated genes (D) in A. baumannii AB5075-UW revealed by RNA-seq. Cells untreated (white dots) and exposed to Spermine NONOate (50 µM) (black dots) for 15 min exposure are represented. Relative gene expression was calculated by normalizing RS01970 Cq values with 16 S Cq values. Each value in the graph represents one independent sample. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.1, without assuming a consistent SD. ***: p-value < 0.01; **: p-value < 0.05.
