Fig. 3
RS01970 protects A. baumannii from NO stress. (A-B) Growth of A. baumannii AB5075-UW (wt, circle) and its ΔRS01970 (triangle) derivative strain. Cells were grown aerobically in LB at 37 °C and 150 rpm. Both, Spermine NONOate (50 µM) (A) and DETA NONOate (500 µM) (B) were added after 1 h (red line) or not (black line). (C-D) Growth of E. coli BW25113 (wt, circle) and its Δhmp derivative strain carrying p0 (pFLAG-CTC empty plasmid, diamond), pHb (pFLAG-CTC expressing RS01970, triangle) or ptrHB (pFLAG-CTC expressing RS01970 truncated protein, square). Cells were grown in LB, under aerobic conditions (C) and microaerobic conditions (D), in the presence of DETA NONOate (500 µM), added at t0. Error bars represent the standard deviation of three independent biological samples.
