Fig. 4

RS01965 is a NsrR-like protein. (A) Schematic representation of genomic organization of A. baumannii AB5075-UWRS01970 locus. RS01950 (dicarboxylate/amino acid: cation symporter), RS01955 (metaldependent hydrolase), RS01960 (YihAfamily ribosome biogenesis GTP-binding protein), RS01965 (Rrf2 family transcriptional regulator), RS01975 (DUF853 domain-containing protein). (B) Multiple protein sequence alignment with selected bacterial NsrR: Pseudomonas aeruginosa (A0A069QAL4), Lactiplantibacillus plantarum (A0A0G9FFQ2), Streptomycescoelicolor (Q9L132), Bacillus subtilis (O07573), Moraxella catarrhalis (B7T0V2), Neisseria meningitidis (X5FBC9), Yersinia pestis (Q0WJT1), Escherichia coli (P0AF63), and Salmonella Typhimurium (A0A0F6BAN2) and RS01965 NsrR (Acinetobacter baumannii). Protein alignment was made using Clustal Omega with custom parameters and colored using Jalview (2.11.3.0). UniProt accession numbers are presented in parentheses. (C) Quantitative real-time PCR (qRT-PCR) of RS01970 gene in the wild-type strain (white circles) and ΔRS01965 strain (black circles). Relative gene expression was calculated by normalizing RS01970 Cq values with 16 S Cq values. Each value in the graph represents one independent sample. Statistical significance was determined using the Holm-Sidak method, with alpha = 0.1, without assuming a consistent SD. **: p-value < 0.05.